Fig. 6

Immediate cued fear memory and secondary contextual fear acquisition deficits in Gde2KO mice. A Schematic of the FC paradigm. Mice are placed in Context 1 on day 1 and at the start of day 2. In trial 1 (fear acquisition), a tone is played before a shock is delivered to the animals 3 separate times during the test. During trial 2 (contextual fear testing), the mice are placed in the same context, but they receive no sound or shock. During trial 3 (Cued memory testing), the mice are placed in a different box, and only the tone is played while no shock is delivered. B, C Percent time WT and Gde2KO mice spent freezing in the ITI (B) and during the CS (C) throughout the fear acquisition trial. No effect of genotype or its interactions were detected (three-way mixed design ANOVA, Ps > 0.05). D, E Percent time spent freezing (D) and dynamics (E) during the contextual fear testing trial. No effect of genotype or its interactions were detected (three-way mixed design ANOVA, Ps > 0.05). F Percent time spent freezing during the CS in trial 3. No effect of genotype or its interactions were detected (three-way mixed design ANOVA, Ps > 0.05). G Quantification of time spent freezing during the ITI between tone deliveries during trial 3 revealed a significant decrease in time spent freezing by Gde2KO mice as the test progressed (ANOVA, effect of genotype (F(1,56) = 6.16, P < 0.016) and genotype x ITI interaction (F(3,168) = 3.0857, P < 0.0288). All graphs are means ± SEM; ns, P > 0.05; and **P < 0.01. See Additional file 2: Table S1 for statistical details. Schematic in A created in BioRender.com