Fig. 2

Effects of early maternal immune activation on microglial activation in the prefrontal cortex (PFC) and hippocampus (HC) regions of offspring. A–D Representative images of immunohistochemical staining for ionized calcium-binding adapter molecule 1 (IBA1) in PFC of rats after early prenatal treatment with Poly I:C (A, C) or vehicle (B, D). Row 1 and row 2: During puberty (row 1) and adulthood (row 2), IBA1-positive cells in the PFC were with round cell bodies and reduced arborizationin the offspring of rats exposed to Poly I:C at gestational day 9; n = 9 for Poly I:C group, n = 7 for vehicle group. E–H Representative images of immunohistochemical staining for IBA1 in HC of rats after early prenatal treatment with Poly I:C (E–G) or vehicle (F–H); n = 13 for Poly I:C group, n = 10 for vehicle group. Row 3 and row 4: IBA1-positive cells in the HC were also with round cell bodies and reduced arborization during puberty (row 3) and adulthood (row 4) in the offspring of rats exposed to Poly I:C at GD9 (×10)