Fig. 4

Histogram of the a freezing bouts and b freezing duration of zebrafish. Control: untreated AB strain zebrafish. Acute: acute treatment with reserpine for 20 min. Model: after acute treatment with reserpine, zebrafish were exposed to system water for 7 days to generate the depression model. Sertraline: after acute treatment with reserpine, zebrafish were exposed to sertraline for 7 days. JWXY: after acute treatment with reserpine, zebrafish were exposed to JWXY capsule for 7 days. The data are expressed as the mean ± S.E.M. and were analysed by one-way ANOVA followed by the Tukey post hoc test. Significance was defined as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001