Fig. 6

BMSCs transplantation decreased the expression of TNF-α and the numbers of inflammatory cells in lungs of rats after MCAO. a, b The intracellular TNF-α in lung (brown signal, thin arrow) was detected by immunohistochemistry. The photos represented the intracellular TNF-α content of lung in BI group (a) and BMSCs group (b). c, d Inflammatory cells in lung of rats in BI group (c) and BMSCs group (d) were detected by HE staining. e Hochest33342 labeled cells (blue) in lung under fluorescence microscope. f Bar graph of mean density of TNF-α in the lung in BI group and BMSCs group. g Numbers of inflammatory cells were calculated in BI group and BMSCs group. (*P < 0.05, n = 5 in each group). a–d, 25 μm; e, 100 μm