Table 1 Primer sequences, PCR conditions and restriction digestion predictions
From: P268S in NOD2 associates with susceptibility to Parkinson’s disease in Chinese population
Polymorphism | Primer sequences (5′ → 3′) | Annealing temperature (°C) | Restriction enzyme | Amplified products length (bp) | Restriction products length (bp) |
|---|---|---|---|---|---|
E46K | F: TGATGTGGGAACAAAGGGGA | 58 | BsaJ I | 747 | 46E allele: 287,460 |
R: GTGTTTCCTGAAATGCACTCTGA | 46K allele: 747 | ||||
A1442P | F: GAGACTAAACTGCTGCTTGC | 58 | Hha l | 801 | 1442A allele: 671,130 |
R: GTAATCTCGTATGGCAGGGA | 1442P allele: 801 | ||||
A350V | F: TCAGTGGTCTTTGCGTCATT | 58 | Mwo I | 302 | 350A allele: 94,208 |
R: CTGTCCTTTTACTCTGCTCCC | 350V allele: 302 | ||||
P268S | F: AGCCCATTGTCTGGTTAGGT | 58 | BamH I | 309 | 268P allele: 309 |
R: ACAGTGTCCGCATCGTCAT | 268S allele: 225,84 | ||||
R702W | F: AGATCACAGCAGCCTTCC | 63 | MspI | 185 | 702R allele: 20, 35, 54, 76 |
R: CACGCTCTTGGCCTCACC | 702W allele: 20, 35, 130 | ||||
G908R | F: CCCAGCTCCTCCCTCTTC | 63 | Hin6I | 380 | 908G allele: 380 |
R: AAGTCTGTAATGTAAAGCCAC | 908R allele: 242, 138 | ||||
1007fs | F: GGCAGAAGCCCTCCTGCAGGGCC | 58 | ApaI | 151 | Wild type allele: 151 |
R: CCTCAAAATTCTGCCATTCC | 1007f allele: 130, 21 | ||||
IVS9* | F: ACTCCTGCGCTTGATTTAGGCAAT | 58 | Xho l | 775 | Wild type allele: 318,457 |
R: TTGGAATTTAGCTGTTCCTTCGGG | IVS9 G → A allele: 775 | ||||
G2385R | F: AGACACTGCTCTCTATATTGCTAAG | 58 | Acc I | 261 | 2385G:261 |
R: CTGAAAAGATGGTGCTGAGAAG | 2385R:77,184 |